王成坤，教授，博士，博士生导师

科学研究方向

基因编辑系统优化；神经精神疾病分子机制研究及药物靶点发现

联系方式

通讯：江苏省南京市江宁区龙眠大道101号学海楼生理学系，邮编211166

电邮：wangchengkun@njmu.edu.cn

简介

王成坤，南京医科大学高层次引进人才、教授、博士研究生导师、独立学术带头人。至今已在Nature Cell Biology、Nucleic Acids Research、Journal of Pineal Research、Cell Discovery等著名国际期刊上发表SCI论文二十余篇，总影响因子逾300，申请并授权专利6项。

研究领域：

综合采用CRISPR/Cas9基因编辑系统、单细胞测序、iPSC定向分化、谱系追踪等方法，开展：

1.    新型基因编辑系统创建及优化；

2.    基因突变关联神经精神疾病分子机制研究及药物靶点发现。

教育背景及工作经历

2023年2月-至今           南京医科大学基础医学院 教授

2018年6月-2023年2月 斯坦福大学（Stanford University）博士后研究员

2013年9月-2018年6月 浙江大学 博士研究生

代表性论文

1.      Chengkun Wang; Yuanhao Qu; Jason K. W. Cheng; Nicholas W. Hughes; Qianhe Zhang; Mengdi Wang; Le Cong; dCas9-based gene editing for cleavage-free genomic knock-in of long sequences, Nature Cell Biology, 2022, 24: 268-278

2.      Chengkun Wang; Jason K W Cheng; Qianhe Zhang; Nicholas W Hughes; Qiong Xia; Monte M Winslow; Le Cong; Microbial single-strand annealing proteins enable CRISPR gene-editing tools with improved knock-in efficiencies and reduced off-target effects, Nucleic Acids Research, 2021, 49(6): 1-16

3.      Chengkun Wang; Qiong Xia; Qianhe Zhang; Yuanhao Qu; Stephen Su; Jason K. W. Cheng; Nicholas W. Hughes; Le Cong; CRISPR-Cas12a System With Synergistic Phage Recombination Proteins for Multiplex Precision Editing in Human Cells, Frontiers in Cell and Developmental Biology, 2022, 9(1): 1-6

4.      Lupeng Ye^#; Chengkun Wang^#(co-first author); Lingjuan Hong; Ninghe Sun; Danyang Chen; Sidi Chen; Feng Han; Programmable DNA repair with CRISPRa/i enhanced homology-directed repair efficiency with a single Cas9, Cell Discovery, 2018, 4(1): 46

5.      Chengkun Wang; Muhammad Masood Ahmed; Quan Jiang; Nan-nan Lu; Chao Tan; Yin-ping Gao; Qaisar Mahmood; Dan-yang Chen; Kohji Fukunaga; Mei Li; Zhong Chen; Christopher S. Wilcox; Yingmei Lu; Zheng-hong Qin; Feng Han; Melatonin ameliorates hypoglycemic stress-induced brain endothelial tight junction injury by inhibiting protein nitration of TP53-induced glycolysis and apoptosis regulator, Journal of Pineal Research, 2017, 63: 1-18

6.      Chengkun Wang; Ravi K. Dinesh; Yuanhao Qu; Arjun Rustagi; Henry Cousins; James Zengel; Yinglong Guo; Taryn Hall; Aimee Beck; Luke Tso; Elif Tokar Erdemic; Kae Tanudtanud; Sheng Ren; Kathy Tzy-Hwa Tzeng; Aaron Wilk; Mengdi Wang; Jan Carette; Russ Altman; Catherine A. Blish; Le Cong; CRISPRa screening with real world evidence identifies potassium channels as neuronal entry factors and druggable targets for SARS-CoV-2, bioRxiv, 2021, 0: 1-25

7.      Chengkun Wang; Juan Cheng; Xingguang Liang; Chao Tan; Quan Jiang; Yongzhou Hu; Yingmei Lu; Kohji Fukunaga; Feng Han; Xin Li; A H2O2-Responsive Theranostic Probe for Endothelial Injury Imaging and Protection, Theranostics, 2017, 7(15): 2803-2813

8.      Chao Tan; Nannan Lu; Chengkun Wang; Danyang Chen; Ninghe Sun; Hang Lyu; Jakob Körbelin; Weixing Shi; Kohji Fukunaga; Yingmei Lu; Feng Han; Endothelium-Derived Semaphorin 3G Regulates Hippocampal Synaptic Structure and Plasticity via Neuropilin-2/PlexinA4, Neuron, 2019, 101(5): 920-937

9.      Xingguang Liang; Chao Tan; Chengkun Wang; Rongrong Tao; Yujie Huang; Kuifen Ma; Kohji Fukunaga; Mingzhu Huang; Feng Han; Myt1l Induced Direct Reprogramming of Pericytes into Cholinergic Neurons, CNS Neurosci Ther., 2018, 24(9): 801-809

10.   Yu Lei^#; Chengkun Wang^#(co-first author); Quan Jiang; Xiaoyi Sun; Yongzhong Du; Yaofeng Zhu; Yingmei Lu; Calpain activation and disturbance of autophagy are induced in cortical neurons in vitro by exposure to HA/β-Ga2O3:Cr3+ nanoparticles, PeerJ, 2018, e6